REAGEN?Beta-lactam  Strip  Test is  a  qualitative and  rapid  lateral flow  assay  designed to detect  beta-lactam  antibiotic residues in raw  commingled cow’s  milk. This  state-of-the-art  test uses a novel  beta-lactam  binding protein  which eliminates  the need  for  aheating block.  This test  is designed  for  point-of-care use  or  reference lab  settings.The unique featuresof the kit are:

l          Rapid strip test method – 7minutes

l         Novel binding protein that requires no heating step

l         High sensitivity

l         High reproducibility

ProcedureOverview

Theassay  uses a competitive colloidal goldbased format. The milk sample  (200 μL)is added to a clear plastic reaction vessel, and used to resuspend thelyophilized reagents to a uniform pink color in the  bottom of the microtiterwells. The  milk  is incubated briefly (3  min) to allow thebeta-lactam binding  protein on  the gold  particles  to engage with  any  beta-lactam antibiotic present in the milk. The test  strip  is then  inserted  into the  sample  well with  the  arrows pointing  downward  initiating capillary flow  up the strip. Any  gold  particles that are  not  complexed with antibiotics  present in themilk  will  bind to the beta-lactam  antibiotic  imprinted at the  Test  line (T-line),  forming a  signal (red line)  at  that position.  If  the beta-lactam  binding  protein on  the  gold particle  has  engaged with antibiotic present in the milk sample, the gold particle will flowpast the T-line and reach the Control line (C-line).  For visual interpretation of the test results,  T-line signalintensity  that  is stronger than the  signal at  the  C-line indicates  a  negative result.  Signal  at the  T-line  which is  equal  or less intense  compared  to the  C-line  indicated the presence  of  beta-lactam antibiotics  in  the milk.  The greater the  reduction in  signal  intensity at  the T-line,  the greater the  concentration  of beta-lactam antibiotics present in the milk.