Fructose/Glucose Assay Kit-REAGEN LLC_

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Fructose/Glucose Assay Kit

MOQ ：4 piece

Lead Time ：7 Days

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Warranty(Year)：1 Year

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Product Description

Product Description

REAGEN^? Fructose/Glucose Assay Kit is acuvette-based enzymatic assay for the determination of Fructose/Glucose acrossa variety of food samples. The kit uses a spectrophotometric, kinetic assay todetect the enzymatic degradation of Fructose/Glucose directly from foodsamples.

The unique features of the kitare:

l Highsensitivity and low detection limit (0.8 ppm)

l Highreproducibility

l A rapid(30 minute), robust enzyme-based assay

ProcedureOverview

REAGEN^? Fructose/Glucose Assay Kit uses a coupled enzymaticreaction to detect glucose and/or fructose in food samples: glucose is detectedby phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). Thiscompound is then used as a substrate by the enzyme glucose-6-phosphatedehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm,is proportional to the level of glucose in the sample. Fructose is detected insimilar manner after it is specifically converted to glucose-6-phosphate(G-6-P) using the enzyme phosphoglucose isomerase (PGI).

The absorbance of each sampleat 340 nm is measured using a spectrophotometer. The concentration of glucosein each sample is then directly determined from the change in absorbance at 340nm within 30 minutes.