Glucose Assay Kit-REAGEN LLC_

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Glucose Assay Kit

MOQ ：4 piece

Lead Time ：7 Days

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Warranty(Year)：1 Year

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Product Description

ProductDescription

REAGEN^? Glucose Assay Kit is a cuvette-basedenzymatic assay for the determination of Glucose across a variety of foodsamples. The kit uses a spectrophotometric, kinetic assay to detect theenzymatic degradation of Glucose directly from food samples.

The unique features of the kit are:

l Highsensitivity and low detection limit (0.8 ppm)

l Highreproducibility

l A rapid(30 minute), robust enzyme-based assay

ProcedureOverview

REAGEN^? Glucose Assay Kit uses a coupled enzymatic reaction todetect glucose and/or fructose in food samples: glucose is detected byphosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). Thiscompound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenaseto convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportionalto the level of glucose in the sample.

The absorbance of each sampleat 340 nm is measured using a spectrophotometer. The concentration of glucosein each sample is then directly determined from the change in absorbance at 340nm within 30 minutes.