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Ascorbic Acid Assay Kit

MOQ :4 piece

Lead Time :7 Days

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Product details

Supply Ability

  • Warranty(Year):1 Year

Product Specifications

Product Description

Product Description

 

REAGEN? Ascorbic Acid Assay Kit is a cuvette-basedenzymatic assay for the determination of Ascorbic Acid across a variety of foodsamples. The kit uses a spectrophotometric, kinetic assay to detect theenzymatic degradation of Ascorbic Acid directly from food samples.

The unique features of the kit are:

 

l       High sensitivity and low detection limit (0.6 ppm)

l         High reproducibility

l         A rapid (25 minute), robust enzyme-based assay.

 

Procedure Overview

 

REAGEN? Ascorbic Acid Assay Kit uses a specificvisible, colorimetric reaction to detect ascorbic acid in food samples:ascorbic acid first reduces an electron carrier (PMS). This compound thenreduces MTT to produce a colored MTT formazan product. This conversion,measured at 578 nm, is proportional to the level of ascorbic acids in thesample. 

 

The absorbance of each sample at 578 nm is measuredusing a spectrophotometer in a cuvette (or plate reader). The concentration ofascorbic acid in each sample is then directly determined from the change inabsorbance at 578 nm within 30 minutes time.

 

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