Nitrate/Nitrite Assay Kit-REAGEN LLC_

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Nitrate/Nitrite Assay Kit

MOQ ：4 piece

Lead Time ：7 Days

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Warranty(Year)：1 Year

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Product Description

ProductDescription

REAGEN^? Nitrate/Nitrite Assay Kit is acuvette-based enzymatic assay for the determination of Nitrate/Nitrite across avariety of food samples. The kit uses a spectrophotometric, kinetic assay todetect the enzymatic degradation of Nitrate/Nitrite directly from food samples.

The unique features of the kitare:

l Highsensitivity and low detection limit (0.02 ppm)

l Highreproducibility

l A rapid(45 minute), robust enzyme-based assay

Procedure Overview

REAGEN^? Nitrate/Nitrite Assay Kit uses acoupled enzymatic reaction scheme to detectnitrate and/or nitrite in food samples: nitrate in the sample is converted bynitrate reductase enzyme to make nitrite. The nitrite then reacts withsulfanilamide and N-(1-naphthyl)-ethylene-diamine to form a red diazo dye. Thisdye, measured at 540 nm, is proportional to the level of nitrate and/or nitritein the sample.

The absorbance of each sampleat 540 nm is measured using a spectrophotometer. The concentration of nitrate/nitrite in each sample is then directly determined from the diazo dyeabsorbance at 540 nm within 45 minutes.